In the present study a simple precise, rapid and reproducible Reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated. The chromatographic separation was carried out by using reverse phase stainless steel column of 4.6×250 mm Hypersil C18 HPLC column with 5 µm (particles packing) held in thermostat at 25°C. The mobile phase consisted of acetonitrile: Phosphate buffer (0.025M) (75/25 v/v), with a flow rate of 0.8 ml/ min and UV detection at 233 nm and injection volume 20 µl was maintained. The retention time was found to be 3.14 min. The different analytical performance parameters such as linearity, accuracy, precision and sensitivity (limit of detection and limit of quantitation) were determined according to international conference on harmonization ICH Q2 (R1) guidelines. The linearity was observed in the range of 10-100 µg/ml with correlation coefficient, r = 0.9981. The proposed method is rapid, cost-effective and can be used as a quality-control tool for routine quantitative analysis of Metformin HCl.
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